[ACE UltraCore SuperC18色譜柱]人參皂甙分析
Conditions
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 100 x 3.0 mm; 2 x 100 x 3.0 mm (coupled); 3 x 100 x 3.0 mm (coupled)
Part Number: CORE-25A-1003U
Mobile Phase:
A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN
Gradient:
|
Time (mins) |
|||
|
100x3.0mm |
200x3.0mm |
300x3.0mm |
%B |
|
- |
0.00 |
0.00 |
5 |
|
0.00 |
0.36 |
0.71 |
5 |
|
10.00 |
20.36 |
30.71 |
70 |
|
11.00 |
22.36 |
33.71 |
95 |
|
13.00 |
26.36 |
39.71 |
95 |
|
14.00 |
28.36 |
42.71 |
5 |
|
22.00 |
44.36 |
66.71 |
5 |
Injection: 2 μL (100 x 3.0 mm); 4 μL (200 x 3.0 mm); 6 μL (300 x 3.0 mm)
Temperature: 80 °C
Detection: UV, 203 nm
Sample: 5 x 75 mg tablets ground to fine powder and extracted with 10.0 mL MeCN/H2O (1:1 v/v) for 15 minutes with ultrasonication. 100 μL supernatant diluted with 300 μL water and filtered using a Whatman Mini-Uniprep syringeless filter
System: Chromaster Ultra Rs
